The Nanodisc system has proven to be a widely applicable means for rendering membrane proteins soluble in aqueous solution and, importantly, in a native-like bilayer environment where they remain monodisperse and active. Some appreciation for the diversity of membrane proteins systems that can be self-assembled into Nanodiscs is evidenced from the Rogue's Gallery below:
We have published several review articles over the past five years that summarize these successes and provide the protocols for use of Nanodiscs. Some of these are available through the PubMed links below:
We remain committed to the widest possible dissemination of the Nanodisc technology, including materials, methods and latest data from our laboratory. The Nanodisc system is now being used by dozens of laboratories around the world that have realized great success and further advanced the technology.
An overview of the Nanodisc methods and guidance for self-assembling your favorite target protein into lipoprotein particles can be obtained through the following link:
You can now:
- obtain membrane scaffold proteins
- obtain pre-assembled Nanodiscs of various sizes and lipid compositions, or
- have your favorite membrane protein assembled into Nanodiscs for a fee.
Send us a query with your specific project needs at BioNanoConLLC@gmail.com for more information.
Academic researchers can acquire limited quantities (< 50 mg) of purified membrane scaffold proteins to try out this technology in their laboratory, as long as supplies are available. However, according to University policy this necessitates execution of a Materials Transfer Agreement administered by the Office of Technology Management at UIUC (contact information provided below). Please direct your queries regarding MTAs and licensing to email@example.com.
Nanodisc technology, and many of its uses, are covered by a broad intellectual property portfolio. Currently, the following patents have issued:
|7,691,414||Membrane scaffold proteins|
|7,662,410||Membrane scaffold proteins and embedded membrane proteins|
|7,622,437||Tissue factor compositions and methods|
|7,592,008||Membrane scaffold proteins|
|7,575,763||Membrane scaffold proteins and tethered membrane proteins|
|7,083,958||Membrane scaffold proteins|
|7,048,949||Membrane scaffold proteins|
The licensing rights to the entire intellectual property suite related to Nanodisc technology is held by the University of Illinois, and not by any private entity. At present, no exclusive licenses have been granted to the private sector, although some non-exclusive licenses are in place. The University’s Office of Technology Management is actively seeking the licensing and commercialization of this technology. Information for licensing Nanodisc technology for commercial, or for Materials Transfer Agreements, please contact:
Office of Technology Management
University of Illinois Urbana-Champaign
319, Ceramics Building MC-243
105 S. Goodwin Avenue
Urbana, IL 61801-2901
Phone: (217) 333 7862
Fax: (217) 265 5530
If you need any further assistance, please do not hesitate to contact Dr. Sligar, our senior technical person for MSP production and Nanodisc assembly (Yelena Grinkova: firstname.lastname@example.org) or our laboratory manager (Aretta Weber: email@example.com).